Published studies show different results about the antibodies detection time with RPA and ELISA. Caroline Pommellet from Biovac designed and conducted an experiment, in which nine 11-week-old chickens were challenged at day 0 by intranasal route with MG and bled for serology every two to three days, starting from the challenge day until day 25. Sera were tested with RPA and ELISA tests. Subsequently, she shares the results and draws conclusions.